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IGNOU MBC-03 (January 2026 – December 2026) Assignment Questions

1. a) Define the following:

i) Mole fraction
ii) Stocks shift
iii) Chromatogram
iv) Half life of radioisotopes
v) Resolving power

b) Discuss the ionization of weak electrolytes with suitable example.

2. a) What is density gradient centrifugation? Discuss briefly any of its method.
b) Write the basic principle and application of NMR Spectroscopy.

3. a) Compare the ultrasonication and freeze thaw methods.
b) Write the basic components and application of Fluorescence spectrophtometry.

4. a) What are Good Laboratory safety rules. Why they are important?
b) Write a brief account note on Bioluminescence and its use in microscopy.

5. (a) How would you prepare the following laboratory solutions?
i) 500 ml of 0.5 M Na2CO3
ii) 100 ml of 0.1 N of NaOH

b) Elaborate the different application of electron microscopy.

6. Differentiate between:
a) Gas Chromatography and HPLC
b) Southern Blotting and Northern blotting

7. a) Describe SDS-PAGE technique.
b) Write an overview on Biological Hazard and its safety aspects.

8. Describe the basic theory, components and applications of thin layer chromatography.
9. a) Explain the steps and application of FISH technique with suitable diagram.

10. Write short notes on the following:
a) Major types of ion sources of mass spectrometer.
b) Ultra-Performance Liquid Chromatography (UPLC)

IGNOU MBC-03 (January 2025 – December 2025) Assignment Questions

1. a) Define the following:

i) pH

ii) Molarity

iii) Numerical aperture

iv) Chemical fixation

v) Rotor

b) Explain the UV-Vis spectrophotometer with suitable diagram.

2. a) What is sub-cellular fractionation? Discuss its process.

b) Write the working principle and basic components of Confocal microscopy.

3. a) Discuss basic theory and application of Atomic force Microscopy.

b) Explain Raman spectroscopy and its applications.

4. a) Define Good Laboratory safety rules. Enlist the chemical safety instructions?

b) Write a brief short note on physical methods of cell disruption.

5. (a) How would you prepare the following laboratory solutions?

i) 250 ml of 0.2 M NaOH

ii) 50 ml of 0.5 N of HCl

b) Explain Bragg’s Law of Diffraction with well labeled diagram.

6. Differentiate between:

a) Paper chromatography and thin layer chromatography

b) FISH and GISH techniques

7. a) Describe isoelectric focusing technique.

b) Discuss the western blotting technique.

8. Describe the basic theory, components and applications of the Gas chromatography.

9. a) Describe the Autoradiography

10. Explain the principle and applications of the following:

a) High-Performance Thin-Layer Chromatography

b) Liquid Chromatography-Mass Spectrometry

MBC-03 Assignments Details

University	:	IGNOU (Indira Gandhi National Open University)
Title	:	Bioanalytical Techniques
Language(s)	:	English
Code	:	MBC-03
Degree	:	MSCBCH
Subject	:	Biochemistry
Course	:	Core Courses (CC)
Author	:	Gullybaba.com Panel
Publisher	:	Gullybaba Publishing House Pvt. Ltd.

IGNOU MBC-03 (Jan 26 - Dec 26, Jan 25 - Dec 25) - Solved Assignment

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